Thiazovivin, 25 mg

A selective small-molecule inhibitor of Rho-associated coiled-coil containing protein kinase (ROCK) supplied by STEMCELL Technologies. The compound is described as a RHO/ROCK pathway inhibitor and as a 90 kDa ribosomal S6 kinase (RSK) inhibitor, and is reported to be effective at 5-fold lower concentrations than another common ROCK inhibitor, Y27632.

Provided as a crystalline solid with a stated purity of ≥ 98%, the substance carries the chemical name N-(phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide (CAS 1226056-71-8), molecular formula C15H13N5OS, and molecular weight 311.4. Reported solubilities include ~0.5 mg/mL in a 1:1 DMSO:PBS solution (pH 7.2), ~1 mg/mL in ethanol, ~14 mg/mL in DMSO, and ~10 mg/mL in DMF. For cell culture use, the stock solution should be diluted into culture medium immediately before use, and the final DMSO concentration should not exceed 0.1%. The product is stable for 12 months from the date of receipt when stored at -20°C and protected from prolonged exposure to light.

In published applications, Thiazovivin promotes survival of human embryonic stem cells during dissociation by stabilizing E-cadherin and improves cell attachment, and supports survival of single human induced pluripotent stem (iPS) cells during transfection for TALEN-mediated genome editing. It is also reported to increase reprogramming efficiency of human somatic cells and cord blood mononuclear cells to iPS cells. The product is classified as hazardous (harmful if swallowed) and is intended for research use only.