Thiamet G, 10 mg

A small molecule from STEMCELL Technologies, Thiamet G selectively inhibits O-GlcNAcase (Ki = 21 nM; Yuzwa et al.), an enzyme that removes O-linked β-N-acetylglucosamine (O-GlcNAc) from proteins. This inhibition of O-GlcNAcase blocks phosphorylation of the tau protein in vivo (Gong & Iqbal). Pathological hyperphosphorylation of the tau protein can aggregate and induce the formation of neurofibrillary tangles, which is a hallmark of Alzheimer's disease (Gong & Iqbal; Yuzwa et al.). The compound is blood-brain barrier permeable and can be used to study the function of O-GlcNAc in the vertebrate brain (Yuzwa et al.).

Thiamet G is supplied as a crystalline solid with a purity of ≥ 98% (CAS Number: 1009816-48-1; Chemical Formula: C9H16N2O4S; Molecular Weight: 248.3 g/mol). The product is stable at -20°C as supplied; prolonged exposure to light should be avoided, and for long-term storage a desiccant is recommended. It remains stable as supplied for 12 months from the date of receipt.

The compound is soluble in DMSO at concentrations ≤ 40 mM and has low solubility in aqueous media. When used as a cell culture supplement, the stock solution should be diluted into culture medium immediately before use, and the final DMSO concentration should be kept below 0.1% to avoid potential cell toxicity. Published applications include decreasing phosphorylation of tau in rats (Yuzwa et al.). The product is for research use only and is classified as hazardous; users should refer to the Safety Data Sheet (SDS).