ThawSTAR CFT2 Transporter, Standalone

From STEMCELL Technologies, this standalone unit is described as a portable solution for the handling and transport of frozen vials retrieved from liquid nitrogen or a -80°C freezer. The manufacturer offers installation, operational, and performance qualification documentation and accessories to facilitate functional testing of the unit.

Manufacturer data compares recovery and viability between the ThawSTAR® CFT2 system and a water bath for three cell types cryopreserved in CryoStor® CS10 and stored in liquid nitrogen for one week. Peripheral blood mononuclear cells (PBMCs) at 3 x 10^7 cells/vial gave a mean live cell recovery of 2.78 x 10^7 cells and mean viability of 92.8% with the CFT2, versus 2.96 x 10^7 cells and 93.9% with a water bath. Frozen monocytes at 2 x 10^7 cells/vial gave 2.05 x 10^7 cells recovery and 95% viability, compared with 1.89 x 10^7 cells and 94.6%. Human pluripotent stem cells (hPSCs) at 1 x 10^6 cells/vial gave 9.05 x 10^5 cells recovery and 83.04% viability, versus 9.35 x 10^5 cells and 82.93%. All assessments used a Nucleoview counter on samples from 3 different donors or cell lines (M001, 1C, and H9 for hPSCs), tested in triplicate.