SepMate-15 500-pack RUO

STEMCELL Technologies SepMate™-15 is a polypropylene tube containing a specialized insert designed to facilitate the isolation of mononuclear cells (MNCs) and peripheral blood mononuclear cells (PBMCs) from human whole blood, bone marrow, or cord blood by density gradient centrifugation. The insert creates a barrier between the density gradient medium and the sample, eliminating the need for careful layering of blood onto the medium.

After centrifugation at 1200 x g for 10 minutes with the brake on, the enriched MNC layer can simply be poured off into a new tube, while the density gradient medium, erythrocytes, and granulocytes remain retained below the insert. The tube is designed to process 0.5 - 5 mL of initial sample and is compatible with density gradient media of 1.077 g/mL such as Lymphoprep™ or Ficoll-Paque™ PLUS. It is also compatible with RosetteSep™ cell enrichment cocktails, allowing isolation of specific cell types by negative selection — including B cells, dendritic cells, monocytes, NK cells, and T cell subsets (CD4+, CD8+, regulatory, and other subsets).

The product is intended for research use only, is manufactured under a cGMP quality management system, and is sterilized by irradiation. Tubes should be stored at ambient temperature until the expiry date on the label and are not intended for use with leukapheresis samples, buffy coat samples, or samples older than 48 hours.