STEMvision Human Cord Blood 7-Day

Colony-forming unit (CFU) assays from human cord blood sources require standardized culture and scoring methods to support hematopoietic stem and progenitor cell research. The STEMvision Human Cord Blood 7-Day assay format from STEMCELL Technologies pairs an automated imaging workflow with a 7 day culture window, enabling consistent enumeration of erythroid and myeloid progenitor colonies.

After plating cells in MethoCult medium and incubating under standard conditions, the instrument's algorithm classifies and counts colony types including CFU-E, BFU-E, CFU-GM, and CFU-GEMM. The protocol is optimized for use with the STEMvision colony counter, reducing operator-to-operator variability compared with manual scoring under a stereomicroscope.

Researchers in cord blood banking, regenerative medicine, and transplantation studies use this 7 day format to evaluate progenitor potential, compare expansion conditions, and document lot-to-lot performance of starting cell populations. Sample processing follows good laboratory practice and the kit is supplied as a single-use assay setup with reagents and consumables sized for the cord blood workflow.