STEMvision Color Human MPB 14-Day

From STEMCELL Technologies, the STEMvision™ Color Human MPB 14-Day analysis package is designed to image and count colonies in hematopoietic colony-forming unit (CFU) assays using human mobilized peripheral blood (MPB) progenitor cells cultured for 14 days in MethoCult™ Optimum. Developed as an "expert CFU counter in a box," the system replaces manual microscope-based colony counting and uses standardized algorithms to reduce inter-operator variation when scoring CFU assays.

The package enables automated identification and counting of total colonies as well as erythroid (BFU-E), myeloid (CFU-G/M/GM), and mixed (CFU-GEMM) colonies. In 14-day MPB CFU assays, STEMvision™ counts are highly correlated to manual inverted-microscope counts, with reported correlation coefficients (R²) of 0.97 for Total Colonies (y=0.97x + 2.44), 0.91 for BFU-E (y=0.96x + 3.74), and 0.95 for CFU-G/M/GM (y=0.96x + 0.90) across n=143 CFU assays. For mixed (CFU-GEMM) colonies, the automated counts typically fall within the range of counts produced by three to seven manual scorers.

Erythroid and mixed colonies that contain hemoglobinized cells are displayed in true red color, with orange and red circles identifying erythroid colonies, yellow circles identifying myeloid colonies, and blue circles identifying mixed colonies in the analyzed images. The package is intended for use as part of the STEMvision™ workflow for CFU assay analysis with human MPB samples.