STEMvision Color Human BM 14-Day

An automated colony-scoring software package from STEMCELL Technologies, designed for use with the STEMvision™ instrument to image and count hematopoietic colonies in 14-day human bone marrow (BM) CFU assays. It replaces manual counting under a microscope by applying standardized algorithms intended to reduce the variability that arises when different people score CFU assays.

The package identifies and counts total colonies, erythroid colonies produced by BFU-E and CFU-E (reported separately or combined), myeloid colonies (CFU-G/M/GM), and mixed colonies (CFU-GEMM). Using the Human BM 14-Day CFU Analysis Package on cryopreserved BM cells cultured for 14 days in MethoCult™ Optimum, automated counts are highly correlated with manual inverted-microscope counts: R² = 0.95 for total colonies (y = 0.88x + 8.79), R² = 0.94 for CFU-G/M/GM (y = 0.92x + 2.55), and R² = 0.89 for CFU-E + BFU-E (y = 0.83x + 6.71), across n = 120 CFU assays. Counts of mixed (CFU-GEMM) colonies typically fall within the range of manual scores obtained by three to seven operators.

The package is used as part of supported workflows alongside STEMCELL Technologies products including meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays and methylcellulose-based media with recombinant cytokines for human cells.