SBI-0206965, 10 mg

This research-grade autophagy inhibitor from STEMCELL Technologies is supplied as 10 mg of lyophilized powder in a single-use vial for research use only. The compound is a selective ATP-competitive inhibitor of ULK1 kinase, the serine/threonine enzyme that initiates autophagosome biogenesis downstream of mTORC1, and published studies also report activity against NUAK1. Investigators commonly treat cell culture models with working concentrations in the 1 to 10 μM range, reconstituting the powder in DMSO to prepare a 10 mM stock solution that is stored at -20 °C protected from light. Applied to western blot readouts of LC3-II and p62, the molecule is used to block canonical autophagy flux and to probe autophagy-dependent survival in tumor cell lines. Peer-reviewed protocols reference the inhibitor in studies of apoptosis, ferroptosis crosstalk, and kinase signaling pathways in vitro.