Pluripotin, 10 mg

A dual small-molecule inhibitor from STEMCELL Technologies, this compound acts on RasGAP and ERK1 to support self-renewal of mouse pluripotent stem cells under defined culture conditions. As reported in the product documentation, it binds Ras GTPase-activating protein (RasGAP) with a Kd of 98 nM and extracellular signal-regulated kinase 1 (ERK1) with a Kd of 212 nM. Inhibiting RasGAP sustains Ras/PI3-kinase signalling that promotes self-renewal, while ERK1 inhibition blocks differentiation signals.

The inhibitor maintains mouse embryonic stem (ES) cells in an undifferentiated, pluripotent state for at least 10 passages without feeder cells, serum, or leukemia inhibitory factor (LIF), and—when combined with LIF—improves the efficiency of ES cell derivation from refractory mouse strains. The manufacturer classifies it as a MEK/ERK pathway inhibitor relevant to workflows involving leukemia inhibitory factor. Reported specifications include a chemical formula of C₂₇H₂₅F₃N₈O₂, a molecular weight of 550.5 g/mol, a purity of ≥ 98%, CAS number 839707-37-8, alternative name SC-1, and activity through the MEK/ERK and PI3K/AKT pathways against ERK and RasGAP.