O6-Benzylguanine, 50 mg

Supplied by STEMCELL Technologies, this small molecule is described as an epigenetic modifier and an efficient irreversible inactivator of the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT), also known as methylguanine DNA methyltransferase (MGMT). The compound inhibits the removal of alkyl groups from guanine residues and from the O6 position of guanine in DNA, restoring DNA integrity. It is an antineoplastic agent used to investigate the role of AGT in carcinogenesis and mutagenesis.

Published applications include enhancing the activity of alkylating agents (nitrosourea, temozolomide, and cyclophosphamide) in malignant glioma xenografts growing in athymic nude mice, and sensitizing CD34+ hematopoietic progenitor cells and a breast cancer cell line to bis-chloroethylnitrosourea (BCNU). The product is a crystalline solid with a stated purity of ≥98% (molecular formula C₁₂H₁₁N₅O; molecular weight 241.2 g/mol; CAS 19916-73-5; NSC 637037).

The compound has low solubility in aqueous media and is soluble in DMSO at ≤120 mM and in absolute ethanol at ≤20 mM. For cell culture use, the stock solution should be diluted into culture medium immediately before use, with final DMSO concentration kept below 0.1% to avoid potential cell toxicity. The product is stable at -20°C as supplied for 12 months from the date of receipt. It should be protected from prolonged exposure to light and stored with a desiccant for long-term storage. This product is hazardous; the Safety Data Sheet (SDS) should be consulted. It is for research use only and is not intended for human or animal diagnostic or therapeutic uses.