(±)-Nutlin-3, 10 mg

STEMCELL Technologies supplies (±)-Nutlin-3, a small-molecule, imidazoline-based p53 pathway activator that acts as a mouse double minute 2 (MDM2) protein antagonist. The compound disrupts the MDM2–p53 interaction with an IC₅₀ of 0.09 µM, relieving MDM2-mediated negative modulation of p53 transcriptional activity and stability. By restoring p53 function, (±)-Nutlin-3 promotes expression of p53-regulated genes and shows potent antiproliferative activity in cells with functional p53, but not in cells with mutated p53.

The product is a crystalline solid with chemical formula C₃₀H₃₀Cl₂N₄O₄, a molecular weight of 581.5 g/mol, a reported purity of greater than 98%, and a CAS number of 548472-68-0. It is soluble in DMSO and absolute ethanol at concentrations up to 40 mM and exhibits low solubility in aqueous media. For example, a 10 mM stock solution in DMSO can be prepared by resuspending 1 mg in 172 μL of DMSO; stock solutions should be prepared fresh before use. For cell-culture applications, stock solutions should be diluted into culture medium immediately before use, and the final DMSO concentration should be kept below 0.1% to avoid potential cell toxicity. The compound is stable for 12 months from the date of receipt when stored at -20 °C, protected from prolonged light exposure, and, for long-term storage, kept with a desiccant.

Published applications include inhibition of proliferation of exponentially growing human skin fibroblasts (IC₅₀ = 2.2 μM) and mouse embryonic fibroblasts (IC₅₀ = 1.3 μM), suppression of growth of established tumor xenografts in mice, induction of G1 cell-cycle arrest in the HCT116 colon carcinoma cell line expressing wild-type p53 and p21, and induction of p53-mediated apoptosis in solid tumor and pediatric acute lymphoblastic leukemia cell lines. The product is supplied for research use only and is classified as hazardous; users should consult the Safety Data Sheet.