MLN4924, 10mg

A cell-permeable small molecule inhibitor of NEDD8-activating enzyme (NAE), this 10 mg vial of MLN4924 from STEMCELL Technologies blocks the neddylation cascade that activates Cullin-RING E3 ubiquitin ligases, stabilizing CRL substrates such as p21, p27, and CDT1 in cultured cells. Researchers commonly apply the compound to in vitro cell culture systems at working concentrations between 0.1 and 1 µM to interrogate cell cycle regulation, DNA damage response pathways, NF-κB signaling, and apoptosis induction in cancer research models.

The reagent is supplied as a lyophilized solid intended for research use only, and is reconstituted in DMSO for stock preparation. It serves as a pharmacological probe for the ubiquitin-proteasome system and has been used to sensitize tumor cell lines to DNA-damaging agents and chemotherapeutics. Routine applications include pilot inhibitor screens, mechanism-of-action studies, and combinatorial treatments in oncology workflows.

Store the powder refrigerated or at -20 °C, protected from light, and prepare single-use DMSO aliquots to limit freeze-thaw cycles. Each vial contains 10 mg of compound, sufficient for multiple replicate experiments in standard multi-well plate formats.