LY411575, 5 mg

STEMCELL Technologies supplies this research-only small molecule as a Notch pathway inhibitor that targets γ-secretase. The compound, LY411575, is described as a cell-permeable γ-secretase inhibitor with an IC₅₀ of 0.14 nM and has been shown to block Notch activation in vitro at 500 µM.

γ-Secretase is a multi-subunit aspartyl protease that regulates signaling pathways by proteolytically cleaving substrates, while Notch is a transmembrane receptor involved in cell fate decisions including proliferation, differentiation, and apoptosis. Published applications include promotion of neuronal differentiation of neural progenitor cells derived from mouse embryonic stem cells, promotion of goblet cell differentiation in mouse intestine and cultured colonic organoids, induction of hair cell differentiation from inner ear stem cells in vitro together with transdifferentiation of supporting cells into hair cells in vivo, and premature differentiation of Her4-positive progenitor cells into neurons in zebrafish. In cancer research, the inhibitor has been reported to induce apoptosis in primary and immortalized Kaposi's sarcoma cells.

The chemical name is (2S)-2-[[(2S)-2-(3,5-difluorophenyl)-2-hydroxyacetyl]amino]-N-[(7S)-5-methyl-6-oxo-7H-benzo[d][1]benzazepin-7-yl]propanamide (CAS 209984-57-6), with chemical formula C₂₆H₂₃F₂N₃O₄ and molecular weight 479.5 g/mol. Reported purity is ≥ 98%, and the material is supplied as a crystalline solid. Reported solubility limits are ≤ 200 µM in DMSO, ≤ 200 µM in absolute ethanol, and ≤ 20 mM in DMF; the compound has low solubility in aqueous media. Storage guidance indicates the product is stable at -20°C as supplied, should be protected from prolonged exposure to light, and remains stable as supplied for 12 months from date of receipt. For cell culture use, stock solution should be diluted into culture medium immediately before use, and a final DMSO concentration above 0.1% should be avoided due to potential cell toxicity.