LY294002, 25 mg

A small-molecule PI3K inhibitor supplied by STEMCELL Technologies, this compound is described as having greater potency and selectivity than quercetin, the structure on which it is based. It inhibits PI3K (IC₅₀ = 1.4 µM) and also shows activity against CK2, but not against PI4K, EGFR, PDGFR, MAPK, PKA, or PKC.

The product is a crystalline solid identified chemically as 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (CAS 154447-36-6), with the molecular formula C₁₉H₁₇NO₃ and a molecular weight of 307.3 g/mol. Reported purity is ≥ 98%. Solubility has been characterized as ~0.5 mg/mL in a 1:1 solution of DMSO:PBS (pH 7.2) and ~16 mg/mL in EtOH, DMF, and DMSO. As a cell-culture supplement, the supplier recommends diluting stock solution into culture medium immediately before use and keeping the final DMSO or absolute ethanol concentration below 0.1% due to potential cell toxicity.

Published applications include suppression of proliferation and self-renewal of mouse embryonic stem cells, promotion of differentiation of mouse ES cells into insulin-producing cells, and inhibition of myotube formation from myoblasts. The product is stable as supplied at -20°C and is intended for research use only.