Ku-0063794, 50 mg

A selective small molecule inhibitor from STEMCELL Technologies that targets the serine-threonine kinase mammalian target of rapamycin (mTOR), blocking both the mTORC1 and mTORC2 complexes with an IC₅₀ of 10 nM. The compound shows greater than 1000-fold specificity over 76 other protein kinases and 7 lipid kinases, including PI3 kinases (García-Martínez et al.).

Supplied as a crystalline solid with a purity of ≥ 98% (CAS Number 938440-64-3; molecular weight 465.5 g/mol). The product is stable at -20°C for 12 months from the date of receipt when protected from prolonged light exposure. For stock preparation, the compound is soluble in DMSO at concentrations up to 4 mM; storage of stock solutions in DMSO at -20°C is generally recommended, with aliquoting to limit freeze-thaw cycles. The compound has low aqueous solubility, so when added to cell culture it should be diluted into medium immediately before use, with final DMSO concentration kept below 0.1% to avoid cell toxicity.

Published applications include extending the lifespan of Toll-like receptor (TLR)-activated dendritic cells by preserving mitochondrial oxidative phosphorylation (Amiel et al., 2014), inducing G1 cell cycle arrest in mouse embryonic fibroblasts and human non-small cell lung carcinoma cell lines (Fei et al., 2013; García-Martínez et al., 2009), inhibiting tumor growth in a renal cell carcinoma xenograft model (Zhang et al., 2013), and reducing keloid volume in an ex vivo organ culture model while inhibiting keloid cell spreading, proliferation, migration, and invasive properties in vitro (Syed et al., 2013). For research use only; refer to the Safety Data Sheet (SDS) for hazard information.