IWP-4, 1 mg

Manufactured by STEMCELL Technologies, this small-molecule research reagent inhibits WNT signaling and secretion by inactivating Porcupine, a membrane-bound O-acyltransferase responsible for palmitoylating WNT proteins, a modification essential for WNT signaling ability and secretion.

The compound impairs WNT pathway activity in vitro with an IC₅₀ value of 25 nM and is classified as a Small Molecules reagent at a stated purity of ≥ 95%. Its alternative name is Inhibitor of Wnt Production-4, with the chemical formula C₂₃H₂₀N₄O₃S₃ and a molecular weight of 496.6 g/mol.

The product promotes cardiomyocyte differentiation in human pluripotent stem cells after treatment with CHIR99021, and promotes cardiomyocyte differentiation in human embryonic stem cells following primitive streak induction with BMP4 and Activin A. It is positioned for defined, xeno-free induction workflows targeting early mesodermal differentiation, with Porcupine identified as the molecular target within the WNT pathway.