Hu PSC Naive qPCR Type 3, 96 wells

Profile naive-state human pluripotent stem cells (PSCs) with this 96-well qPCR array from STEMCELL Technologies, supplied as a plate of lyophilized, pre-dispensed gene-specific primers. The Type 3 panel targets naive pluripotency-associated transcripts and is used to characterize the ground-state identity of human PSCs maintained under naive culture conditions.

Each well of the dried-down plate contains primers for a single human gene target, supporting SYBR Green-based quantitative PCR on real-time instruments such as the Applied Biosystems QuantStudio and Bio-Rad CFX systems. Primer pairs correspond to human-specific sequences with no significant cross-reactivity with mouse orthologous transcripts, allowing direct comparison across species-matched controls. Workflow combines RNA extraction, reverse transcription, qPCR master mix addition, plate loading, and standard cycling protocols on a real-time PCR instrument.

Applications include monitoring primed-to-naive conversion of human PSCs, validating newly established naive PSC lines, and benchmarking naive versus primed pluripotency gene expression for research use only. The format supports 96-well real-time PCR blocks and integrates into routine pluripotency characterization workflows in stem cell research labs.