HPI-1, 25 mg

A small-molecule antagonist of the Smoothened (Smo) receptor, HPI-1 is used by researchers to selectively block Hedgehog (Hh) signaling in cultured mammalian cells. Supplied by STEMCELL Technologies as a 25 mg vial of lyophilized powder, this inhibitor is commonly applied to studies of embryonic development, neural patterning, and stem cell lineage specification, where controlled suppression of the Hh pathway helps clarify its role in cell fate decisions.

The compound is soluble in DMSO and is typically added to culture medium at working concentrations between 1 and 10 µM. Investigators use it to prevent spontaneous Hedgehog-driven differentiation of neural progenitors, to probe glioma and medulloblastoma cell behavior, and to evaluate downstream Gli transcription factor activity. Store the powder at -20 °C, protected from light, and avoid repeated freeze-thaw cycles by preparing single-use aliquots in DMSO. For research use only; not intended for diagnostic or therapeutic applications.