Brefeldin A, 25 mg

STEMCELL Technologies supplies Brefeldin A as a research reagent. The manufacturer describes the compound as interfering with protein trafficking, and notes effects on CRISPR gene editing and cancer cell survival.

Broader literature describes Brefeldin A as a lactone antibiotic and inhibitor of protein transport that targets intracellular vesicular trafficking. It is reported to disrupt Golgi complex structure, block endoplasmic reticulum-to-Golgi transport, and trigger endoplasmic reticulum stress, ultimately inducing cancer cell differentiation and apoptosis. Additional reported activities include inhibition of autophagy and mitophagy and use as a small-molecule enhancer to improve CRISPR-mediated homology-directed repair efficiency. Its mechanism of action has also been associated with ARF-GEF inhibition in immune cell research.

Chemical identifiers for Brefeldin A include CAS Number 20350-15-6, molecular formula C16H24O4, and molecular weight 280.36. Solubility in DMSO is reported at 56 mg/mL (199.74 mM), with a recommended stock concentration of 10 mM in DMSO. The compound is reported as insoluble in water and ethanol.