BIO, 5 mg

BIO is a highly selective, cell-permeable small-molecule inhibitor that targets glycogen synthase kinase-3 (GSK-3) and is supplied at 5 mg per vial by STEMCELL Technologies for use in stem cell biology research.

This indirubin-derived compound modulates the canonical Wnt/β-catenin signaling pathway, making it a workhorse tool for maintaining pluripotency in human and mouse embryonic stem cells, enhancing reprogramming efficiency during the generation of induced pluripotent stem cells (iPSCs), and directing lineage-specific differentiation in cell culture workflows.

Researchers commonly apply BIO at working concentrations in the low micromolar range to block GSK-3β activity, stabilize β-catenin, and promote self-renewal of stem cell populations without exogenous growth factors. Stock solutions are typically prepared in DMSO and stored protected from light at -20°C, where the material remains stable through multiple freeze-thaw cycles when aliquoted into single-use vials.

Each lot is backed by STEMCELL Technologies quality-control documentation, making BIO appropriate for research use only in applications ranging from pathway analysis and small-molecule screening to disease modeling involving Wnt signaling dysregulation.