Anti-Rabbit IgG (H+L) (AF488)

Secondary antibodies raised in donkey and conjugated to Alexa Fluor 488 enable sensitive detection of rabbit IgG in immunofluorescence and flow cytometry. This 100 µl single-vial liquid reagent from Antibodies.com targets both the heavy and light chains of rabbit IgG, providing broad coverage across rabbit primary antibody subclasses. AF488 is excited efficiently by the 488 nm blue laser line and emits in the green region of the visible spectrum, aligning with standard FITC filter sets on fluorescence microscopes and cytometers. Store refrigerated and protected from light to preserve the conjugate's fluorescence, and minimize freeze-thaw cycles that can reduce binding activity. The antibody is supplied at a concentration suitable for further dilution in common assay buffers, with working ranges commonly between 1:200 and 1:1000 depending on sample type and instrument setup. Intended for research use only.