Anti-IgG Antibody (HRP)

The Antibodies.com Anti-IgG Antibody (HRP) is a mouse secondary reagent that selectively recognizes the heavy chain (H chain) region of rat IgG, enabling specific detection of intact immunoglobulin molecules without cross-binding to free light chains. Supplied as a 100 µg liquid formulation, this horseradish peroxidase–conjugated antibody performs in Western blot, ELISA, and immunohistochemistry workflows, providing enzymatic signal with chromogenic substrates such as TMB or chemiluminescent ECL detection. Recommended starting dilutions typically range from 1:1000 to 1:10000 depending on assay format. Store at 4°C short term; for longer storage, aliquot and freeze at -20°C, avoiding repeated freeze-thaw cycles. Researchers should validate reactivity in their specific sample type before large-scale use.