AG-490, 10 mg

A reversible Janus kinase (JAK) inhibitor supplied as a 10 mg single-use vial, AG-490 from STEMCELL Technologies is a benchmark research tool for cell signaling and oncology investigations. This small-molecule compound, chemically known as Tyrphostin AG-490 (alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide), selectively blocks JAK2 and JAK3 tyrosine kinase activity, disrupting downstream STAT3 phosphorylation and the transcriptional cascade normally driven by cytokines such as IL-6 and erythropoietin. Researchers employ it in cell culture experiments to probe JAK/STAT pathway contributions in leukemia, multiple myeloma, and inflammatory disease models. The powder dissolves readily in DMSO, with published working concentrations typically in the 1 to 100 micromolar range depending on the cellular system. Documented applications include apoptosis assays, proliferation studies, western blot analysis of phospho-STAT3, and flow cytometry investigations of cytokine-driven immune cell activation. Store the material desiccated and protect from light; aliquot stock solutions in DMSO to limit freeze-thaw cycles and preserve activity across extended experimental timelines.