A 83-01, 150 mg

STEMCELL Technologies supplies this small molecule as a potent inhibitor of activin receptor-like kinases (ALK), targeting ALK5 (type I transforming growth factor-β receptor), ALK4 (type IB activin receptor), and ALK7 (type I NODAL receptor) with IC50 values of 12 nM, 45 nM, and 7.5 nM, respectively. It is described as an Activin/NODAL/TGF-β pathway inhibitor and disrupts epithelial-to-mesenchymal transition through inhibition of the SMAD signaling pathway.

The compound is provided as a crystalline solid with a purity of ≥ 95%. Its molecular formula is C25H19N5S, with a molecular weight of 421.5 g/mol and a CAS number of 909910-43-6. The chemical name is 3-(6-methylpyridin-2-yl)-N-phenyl-4-quinolin-2-ylpyrazole-1-carbothioamide. It is soluble in DMSO at concentrations ≤ 45 mM and has low solubility in aqueous media. As supplied, the product is stable at -20°C and should be protected from prolonged exposure to light; for long-term storage, store with a desiccant. Stable as supplied for 12 months from the date of receipt. When used as a cell culture supplement, the stock solution should be diluted into culture medium immediately before use, with final DMSO concentration kept below 0.1% to avoid potential cell toxicity.

Published applications include facilitating the conversion of epiblast stem cells to the naïve pluripotent state, enabling OCT4-mediated reprogramming when combined with PD0325901, and inhibiting mesoderm and endoderm specification. For research use only; not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.