A 83-01, 10 mg

STEMCELL Technologies supplies this small molecule as a research-use-only Activin/NODAL/TGF-β pathway inhibitor that targets ALK5, ALK4, and ALK7. It is provided as a crystalline solid with a reported purity of ≥ 95%, a molecular weight of 421.5 g/mol, and the chemical formula C25H19N5S. The compound is stable as supplied for 12 months from the date of receipt when stored at -20°C, protected from prolonged light exposure, and kept with a desiccant for long-term storage. Stock solutions are typically prepared in DMSO, and the compound has low solubility in aqueous media, so it should be diluted into culture medium immediately before use as a cell culture supplement, with final DMSO concentration above 0.1% avoided due to potential cell toxicity.

Functional characterization shows that the compound is a potent inhibitor of activin receptor-like kinase (ALK), with IC50 = 12, 45, and 7.5 nM for ALK5 (type I transforming growth factor-β receptor), ALK4 (type IB activin receptor), and ALK7 (type I NODAL receptor), respectively, as reported by Tojo et al. Published applications include facilitating the conversion of epiblast stem cells to the naïve pluripotent state, enabling OCT4-mediated reprogramming in combination with PD0325901, inhibiting mesoderm and endoderm specification, and disrupting epithelial-to-mesenchymal transition through inhibition of the SMAD signaling pathway.

As with other small molecules from STEMCELL Technologies, the product is for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated. The compound is classified as hazardous, and users are referred to the Safety Data Sheet.