3-Methyladenine, 50mg

A widely used small-molecule autophagy inhibitor in cell biology and biomedical research, 3-methyladenine (molecular weight ~149.15 g/mol, CAS 5142-23-4) from STEMCELL Technologies is supplied as a 50 mg vial of crystalline solid. The compound selectively blocks class III phosphatidylinositol 3-kinase (Vps34) activity, halting autophagosome formation in cultured mammalian cells.

Researchers commonly prepare stock solutions in sterile water or DMSO and apply the inhibitor at working concentrations of 5 to 10 mM for short-term in vitro treatments. It is routinely paired with nutrient-starvation protocols (EBSS or HBSS exposure) to confirm autophagic flux and combined with downstream readouts such as LC3-II turnover, p62/SQSTM1 accumulation, and tandem RFP-GFP-LC3 reporter imaging. The reagent has been used in neuronal, hepatic, and tumor cell models to probe the role of autophagy in apoptosis, ER stress, and disease mechanisms.

Store the vial tightly sealed, protected from light, at room temperature or refrigerated. For solution preparation, aliquot stocks to limit freeze-thaw cycles. Intended for research use only, not for diagnostic or therapeutic applications.