Rotor-GeneQ 5plexHRM Platf. System PRV-1

Built for quantitative PCR and high-resolution melt analysis, this real-time cycler from QIAGEN uses a centrifugal rotor instead of a traditional heated block, eliminating well-to-well temperature variation across its 72-tube rotor. The 5plex optical configuration resolves five fluorescent channels simultaneously, while a dedicated HRM channel distinguishes sequence variants down to single nucleotide differences.

Laboratories deploy the platform for mutation scanning, SNP genotyping, gene expression quantification, and pathogen detection in clinical microbiology and food safety workflows. The system supports 0.2 mL strip tubes, allowing flexible throughput and reducing cross-contamination risk. A typical reaction uses 10-25 uL sample volume, and the rotating chamber design reaches uniform thermal conditions across all positions within seconds, accelerating cycling.

Designated for PRV-1 screening as configured, the instrument integrates with QIAGEN's PCR reagent portfolio, including QuantiTect and Type-it assays, and runs HRM genotyping, absolute quantification, and melt curve analysis through built-in software without requiring additional modules. Applications include veterinary pathogen screening, resistance marker detection, and research use only protocols where reproducible melt curves are critical.