QIAvac Multiwell

QIAGEN's QIAvac Multiwell is a vacuum manifold designed for efficient processing of 96-well purification plates conforming to the Society for Biomolecular Screening (SBS) standard, including the plates used with the DirectPrep 96 Miniprep Kit and the MinElute 96 UF PCR Purification Kit. The 96-well purification plate fits precisely into the top of the unit, and samples and buffers are drawn by vacuum pressure through the purification plate membrane into a collection vessel or waste tray located in the base.

The manifold enables parallel sample processing and reduces sample handling. Internal components are easily cleaned by separating the top plate from the base. For optimal results, QIAGEN recommends using the unit together with the QIAvac Connecting system and a vacuum pump. An Elution Microtube adapter is available to securely hold a rack of Elution Microtubes RS, and a Microplate adapter is required to secure 96-well microplates within the manifold.

According to the MinElute 96 UF PCR Purification Kit protocol, vacuum should be maintained at –800 mbar for 5 minutes or until the wells are completely dry; if the PCR volume exceeds 50 µl, a longer vacuum time is needed. Processing PCR sample volumes larger than 150 µl may lead to increased processing time and incomplete primer removal.