QIAquant 96 5plex (230 V)

A high-performance real-time PCR thermal cycler from QIAGEN, designed for molecular biology applications including expression analysis, genotyping, and pathogen detection. The 96-well block with 5plex detection supports multiplex analysis of up to five different targets in a single run.

The instrument features a silver sample block with gold coating for outstanding thermal conductivity and a motorized heated lid adjustable from 30°C to 110°C. It is compatible with standard SBS plates, 8-well strips, and 0.2 ml individual vessels with optical lids. The fast fiber-optic shuttle system reads out the entire plate in 6 seconds regardless of the number of filters used, and a complete cycling run can be completed in 30 minutes. Operation is via touchscreen and/or PC.

Technical capabilities include a temperature range of 3°C – 99°C with temperature accuracy of 0.1°C, a temperature gradient of 40°C (0.1°C steps), max. heating rate of 8°C/s (av. 7°C/s), max. cooling rate of 6°C/s (av. 5.5°C/s), and temperature uniformity of ±0.15°C at 55°C. The photomultiplier (PMT) detector paired with four high-intensity LEDs (blue, green, white, red) achieves a sensitivity of 1 nmol/l FAM at 30 µl sample volume and a dynamic range of 10 log stages. Five color modules enable multiplexing across Blue, Green, Orange, Red, and NIR channels. Analysis methods include absolute quantification, relative quantification, ΔΔCt, allelic discrimination, efficiency calculation, DNA melting curves, and POS/NEG end-point analysis, with export functions for Excel, CSV, LIMS, GenEx, and qBase.