QIAquant 96 5plex (115V) System

Engineered by QIAGEN for laboratories requiring multiplexed nucleic acid quantification, this 115V real-time PCR thermal cycler interrogates up to five targets per well on a silver sample block with gold coating. A patented Fiber-Optic Shuttle System scans each well using five high-intensity LEDs (blue, green, white, red, and far-red) and a photomultiplier detector, capturing fluorescence through five color modules at 455/515 nm, 520/560 nm, 580/620 nm, 633/680 nm, and 660/710 nm.

The 96-well block accepts sample volumes from 5 to 100 µl (10–80 µl recommended) and runs a full five-color plate read in approximately 6 seconds, with a Peltier-driven temperature gradient of 40 °C and a maximum heating rate of 8 °C/s. Sensitivity is rated at 1 nmol/l FAM at 30 µl sample volume, with dynamic range across 10 log stages and block uniformity of ±0.15 °C at 55 °C, ±0.25 °C at 72 °C, and ±0.50 °C at 95 °C after 15 seconds.

The platform supports probe-based chemistries plus SYBR Green and EvaGreen detection, pairing with QIAquant Software for absolute quantification, ΔΔCt, allelic discrimination, efficiency calculation, and DNA melt curve analysis with built-in cross-talk compensation. LIMS integration enables traceable data export, while the standalone touchscreen simplifies plate setup for pathogen detection, viral load quantification, SNP genotyping, residual DNA testing in biopharmaceutical workflows, and gene expression analysis in cell culture research. Installation, training, and preventive maintenance are coordinated through QIAGEN's global service organization.