QIAquant 96 2plex (115V) System

This dual-channel real-time PCR thermal cycler from QIAGEN supports quantitative nucleic acid analysis across a 96-position block, enabling two-target multiplex reactions per well. It serves gene expression studies, pathogen detection workflows, and SNP genotyping assays in research laboratories.

The instrument incorporates a gradient-enabled thermal block that allows optimization of annealing temperatures across 12 columns, accelerating assay development. LED-based excitation paired with photodiode detection provides sensitive fluorescence readout without the maintenance demands of laser-based optics. An integrated touchscreen interface enables standalone protocol setup, while LAN connectivity links the system to laboratory information pipelines.

The 115V configuration matches North American laboratory power standards. Compatible consumables include standard 0.2 mL tubes, strip tubes with optical sealing films, and microplates. Recommended reaction volumes fall within the 10 to 50 uL range, with thermal ramp rates near 4 °C/sec for both heating and cooling steps.

Chemistry compatibility spans intercalating dye methods such as SYBR Green I as well as probe-based detection, including standard 5'-nuclease assays. Bundled software supports melt curve analysis, absolute quantification with standard curves, relative expression normalization across multiple reference genes, and allelic discrimination genotyping. The unit is delivered as a benchtop instrument ready for PCR setup, and storage at room temperature between runs keeps the optics stable.