PyroMark Q96 Vacuum Workstation UK

QIAGEN's PyroMark Q96 Vacuum Workstation serves as the dedicated preparation station for the PyroMark Q96 pyrosequencing system, enabling researchers to convert biotinylated PCR products into single-stranded DNA templates prior to sequence analysis. The unit uses a controlled vacuum manifold to capture streptavidin-coated Sepharose beads, allowing users to wash, denature, and anneal templates in a streamlined batch process compatible with 96-well plates.

In a typical workflow, amplified PCR product is mixed with the bead suspension, drawn through the workstation's filter probes, and treated sequentially with denaturation solution and annealing buffer. The result is a template-ready plate that transfers directly to the Q96 instrument for nucleotide incorporation readout via pyrosequencing chemistry. A standard sample volume of 25 µL PCR product combined with 3 µL streptavidin bead suspension is loaded per well as the starting material. This preparation approach supports applications in quantitative methylation analysis, SNP genotyping, microbial resistance typing, and verification of next-generation sequencing library tags.

Routine care includes cleaning the probe tips with ultrapure water after each run and inspecting the vacuum seals periodically to maintain consistent aspiration force. The workstation is engineered for benchtop placement beside the Q96 plate reader, with UK power configuration, and integrates with QIAGEN's PyroMark assay design software for downstream analysis. Each workstation ships ready for connection to a laboratory vacuum source and is intended for research use only, supporting laboratories performing mutation detection and epigenetic studies where throughput and template purity matter most.