5-plex HRM Realtime Auto. PCR Workflow

Simultaneous amplification of five molecular targets in a single closed-tube reaction defines QIAGEN's 5-plex HRM Real-time PCR workflow, which combines rotary real-time cycling with High Resolution Melt analysis for mutation scanning and genotyping.

The platform runs five optical channels, eliminating well-to-well variation through its centrifugal design, and applies validated thermal protocols to discriminate SNPs, methylation changes, and pathogen variants at single-base resolution.

HRM temperature ramping spans a 65 degrees C to 95 degrees C gradient with 0.02 degrees C steps, supporting research use only assays in infectious disease identification and microbial genotyping.

Reagent kits ship in a pack of tubes containing master mix, buffer, and enzyme components for storage at -20 degrees C, and integrate with QIAGEN's automated sample preparation and downstream analysis modules.