Shz-1, 10 mg

A cell-permeable, small-molecule inhibitor of the Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP-1), Shz-1 is supplied as 10 mg of lyophilized powder for research use only. From STEMCELL Technologies, this tool compound is widely used to probe phosphatase-dependent signaling cascades in immune cells, including mast cell degranulation, FcεRI-mediated activation, and downstream cytokine release pathways. By blocking SHP-1 catalytic activity, the compound modulates early signaling events downstream of immunoreceptor engagement, allowing investigators to dissect the contribution of this phosphatase to cellular responses in vitro.

Shz-1 is typically dissolved in DMSO to prepare a stock solution and applied to cultured cells at low micromolar working concentrations, depending on the assay system. Researchers employ this inhibitor in western blot, flow cytometry, and ELISA readouts to measure enhanced phosphorylation of key substrates such as Syk, LAT, and PLCγ following immune receptor stimulation. Because of its role in negative regulation of immune receptor signaling, Shz-1 is also investigated in studies of allergy, inflammation, and Fc receptor biology. The 10 mg format provides sufficient material for multiple replicate experiments and is supplied in a single vial for convenient storage at -20 °C, protected from light and moisture.