CoV Protease Substrate-2 EDANS,1000tests

Fluorogenic peptide substrate enabling continuous monitoring of coronavirus protease activity in biochemical and cell-based assay systems. Each vial provides enough reagent for 1000 individual reactions, supporting high-throughput screening campaigns that identify small-molecule inhibitors of viral 3C-like protease (3CLpro) and papain-like protease (PLpro). The EDANS donor fluorophore emits a strong signal upon cleavage of the peptide backbone, allowing real-time kinetic readouts on standard fluorescence plate readers with excitation near 340 nm and emission around 490 nm. STEMCELL Technologies supplies this substrate lyophilized and recommends storage at -20 °C protected from light to preserve fluorophore integrity across multiple freeze-thaw cycles. The reagent is compatible with in vitro protease activity assays, inhibitor dose-response profiling, and purification QC workflows for recombinant CoV protease preparations, making it a practical choice for antiviral drug discovery research.