CoV Protease Substrate-1 EDANS,100tests

Fluorescence resonance energy transfer (FRET) substrates are foundational reagents in protease research, and this EDANS-labeled peptide from STEMCELL Technologies offers continuous, real-time monitoring of coronavirus protease activity such as SARS-CoV-2 Mpro/3CLpro cleavage. The peptide incorporates the EDANS fluorophore paired with a DABCYL quencher, meaning fluorescence is suppressed when the substrate is intact and rises proportionally upon proteolytic cleavage, enabling kinetic measurement of enzymatic activity.

The substrate supports research use only workflows in antiviral drug discovery, protease inhibitor screening, and biochemical characterization of viral proteases. Supplied as a single vial sized for 100 tests, it integrates directly into standard fluorescence plate reader assays. Typical detection uses an excitation wavelength near 340 nm and emission near 490 nm, with the signal increase tracked over time to determine reaction rates and inhibitor potency.

For stability, store frozen at -20 degrees C, protect from light, and aliquot upon first use to avoid repeated freeze-thaw cycles. The vial format retains activity through multiple experimental sessions when handled under these conditions, making it a practical reagent for both routine screening and detailed enzymatic characterization of coronavirus proteases in vitro.