Rt IgG2b,k Ctrl, RTK4530, PE, 100tests

A rat IgG2b, kappa isotype control antibody, clone RTK4530, conjugated to PE, is offered by STEMCELL Technologies. It is suitable for use as an isotype-matched control to estimate the degree of non-specific binding by an antigen-specific antibody, ideally matching the same heavy chain subclass (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being employed. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

The RTK4530 clone has been verified for assessing non-specific binding to cells in flow cytometry and immunofluorescence microscopy applications, including surface and intracellular staining. Listed applications include flow cytometry, functional assays, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blot. The area of interest is immunology, and the host species is rat.