Rt IgG2b,k Ctrl, RTK4530, FI, 100tests

This FITC-conjugated monoclonal antibody (Clone RTK4530, rat IgG2b, kappa) from STEMCELL Technologies is intended for use as an isotype-matched control in flow cytometry applications. It is suitable for estimating the degree of non-specific binding by an antigen-specific antibody, including binding to Fc receptors or other cell components. The clone recognizes keyhole limpet hemocyanin (KLH) and has unknown binding specificity, having been screened on activated, resting, live, and fixed tissues from mouse, rat, human, and non-human primates.

The antibody was raised against trinitrophenol + KLH and purified by affinity chromatography before conjugation with FITC under optimal conditions; the resulting solution is free of unconjugated FITC. It is supplied in a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide; some presentations additionally include 0.2% (w/v) bovine serum albumin. The product should be stored undiluted at 2 - 8 °C, protected from prolonged exposure to light, and not frozen. According to the manufacturer's Safety Data Sheet, the substance is not classified as hazardous under the Globally Harmonized System (GHS).