Rt IgG2b,k Ctrl, RTK4530, BT, 200µg

From STEMCELL Technologies, this Rat IgG2b, kappa Isotype Control Antibody (Clone RTK4530) is a biotin-conjugated rat monoclonal antibody intended for use as an isotype-matched control to estimate the degree of non-specific binding by an antigen-specific antibody. The antibody has been screened on a variety of activated, resting, live, and fixed tissues from several species, including mouse, rat, human, and non-human primates, and recognises keyhole limpet hemocyanin with unknown binding specificity. Ideally, the isotype control shares the same subclass of heavy chain and light chain as the specific antibody being employed, and if a conjugated antibody is used, the isotype control should be conjugated to the same molecule. Use of an appropriate isotype control helps confirm specificity and indicates non-specific binding that may result from interactions with Fc receptors or other cell components.

The RTK4530 clone is verified for use as an isotype control antibody for assessing non-specific binding to cells in flow cytometry applications, with reported use in flow cytometry. The immunogen is Trinitrophenol + KLH. The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions, with the solution free of unconjugated biotin. It is supplied at 0.5 mg/mL in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The product is stable at 2 - 8 °C when stored undiluted and should not be frozen. The suggested use is at concentrations comparable to those of the specific antibody of interest.

Flow cytometry analysis of C57BL/6 mouse splenocytes labelled with the biotin-conjugated RTK4530 antibody followed by streptavidin (SAV) APC illustrates its use as an isotype control, with comparison to a rat IgG2b, kappa positive control antibody.