Rt IgG1,k Ctrl, RTK2071, PE, 25µg

Intended for use as an isotype-matched control, this rat IgG1, kappa antibody reagent (clone RTK2071) from STEMCELL Technologies is used in experiments to estimate the degree of non-specific binding by an antigen-specific antibody. For a meaningful comparison, the control should share the same heavy chain subclass and light chain type as the specific antibody being employed, and—when a conjugated primary is used—the control should be conjugated to the same molecule, such as the same fluorochrome.

Appropriate use of a matching isotype control helps confirm the specificity of the antigen-specific antibody and reveals non-specific binding that may result from interactions with Fc receptors or other cellular components. The RTK2071 clone has been verified as an isotype control for assessing non-specific binding to cells in flow cytometry and in immunofluorescence microscopy applications, including both surface and intracellular staining.

The source material additionally lists functional assays, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blot among the applications associated with this reagent. The host species is rat and the immunoglobulin isotype is IgG1, kappa, with conjugation options referenced as PE, FITC, Alexa Fluor 488, APC, biotin, and unconjugated formats.