Rt IgG1,k Ctrl, RTK2071, FI, 200µg

This FITC-conjugated rat monoclonal antibody from STEMCELL Technologies is intended for use as an isotype-matched control antibody to help estimate the degree of non-specific binding by an antigen-specific antibody in several applications. The antibody (IgG1, kappa, Clone RTK2071) recognizes keyhole limpet hemocyanin (KLH) and has unknown binding specificity, having been screened on a variety of activated, resting, live, and fixed tissues from several species, including mouse, rat, human, and non-human primates. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in flow cytometry applications. The immunogen used was trinitrophenol + KLH. The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions, and the solution is free of unconjugated FITC. It is supplied as a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The product is stable at 2 - 8°C when stored undiluted; it should not be frozen and should be protected from prolonged exposure to light. The suggested use of this antibody is at concentrations comparable to those of the specific antibody of interest.

According to the Safety Data Sheet, this laboratory reagent is a clear liquid and is not classified as a hazardous substance or mixture according to the Globally Harmonized System (GHS) or the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200). Products from STEMCELL Technologies are for research use only and are not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.