Rotor-Gene SYBR Green PCR Demo Kit (80)

Intended to demonstrate the performance of the Rotor-Gene Q real-time PCR cycler, this demo kit from QIAGEN provides all necessary reaction components to quantify a genomic DNA target by SYBR Green-based real-time PCR. Contents include prediluted genomic DNA standards, 2 unknown samples, an optimized master mix, and a primer pair specific for the human G protein-coupled estrogen receptor 1 (GPER) gene. Using the kit, the reliability, reproducibility, and sensitivity of gene quantification with the Rotor-Gene Q can be evaluated.

The Rotor-Gene Q employs a unique centrifugal rotary design in which PCR tubes are placed into a rotor that spins past a single excitation light source and a single detector in a chamber of moving air. This configuration minimizes optical and temperature variation between tubes, enabling high precision in real-time PCR quantification, with continuous rotation at 400 rpm supporting high-speed data acquisition. Highly specific amplification is achieved through a balanced combination of ions that minimizes nonspecific primer annealing, while fast cycling is enabled by Q-Bond, a PCR additive that allows cycler run times as low as 45 minutes.

The accompanying handbook contains 3 protocols: manual reaction setup, automated reaction setup using the QIAgility, and real-time PCR on the Rotor-Gene Q. The QIAgility is a compact benchtop instrument providing rapid, high-precision PCR setup that reduces the risk of human error and complements the Rotor-Gene Q and Rotor-Gene Kits for dispensing liquids into tubes, strip tubes, and Rotor-Discs. A standard curve is generated from CT values of standards at 2000, 1000, 500, 250, and 125 copies (each analyzed in quadruplicate), then used to determine copy numbers of 2 unknown samples at 500 and 250 copies (24 replicates of each). With 4 no-template control (NTC) reactions, 72 reactions are run simultaneously on the Rotor-Gene Q. The kit is also compatible with the Rotor-Gene 3000 and Rotor-Gene 6000.