RosetteSep Human T Enrichment, 10 mL

Designed by STEMCELL Technologies, this immunodensity negative selection cocktail isolates untouched T cells directly from human whole blood. The RosetteSep™ antibody cocktail crosslinks unwanted cells in human whole blood to multiple red blood cells, forming immunorosettes. During centrifugation over a buoyant density medium such as Lymphoprep™ (density 1.077 g/mL), the unwanted rosetted cells pellet along with the free red blood cells. Desired T cells remain unlabeled with antibodies and are collected as a highly enriched population at the interface between the plasma and the density gradient medium, making them immediately available for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

The product is fast and easy to use, requires no special equipment or training, and can be combined with SepMate™ tubes for consistent, high-throughput sample processing. It is suitable for use with whole blood and buffy coat samples and supports research workflows in immunology. The cocktail is supplied as a combination of monoclonal antibodies in PBS and uses tetrameric antibody complexes recognizing non-T cells and glycophorin A on red blood cells.

Starting with fresh human whole blood, the typical CD3+ cell content of the enriched fraction ranges from 90% to 97%. For optimal performance, whole peripheral blood should be used within 24 hours of collection and stored at room temperature (15 - 25°C). The cocktail should be stored at 2°C to 8°C and not frozen; it remains stable until the expiry date on the label.