RosetteSep Human CD8+ T Cells, 0.5mL

A negative-selection cell isolation platform enriches CD8+ T lymphocytes directly from human whole blood in a single centrifugation step. The supplied antibody cocktail crosslinks unwanted leukocytes to red blood cells, generating dense immunorosettes that sediment through a density gradient interface, leaving CD8+ T cells suspended in the upper plasma layer.

The 0.5 mL vial contains a tetrameric antibody complex mixture directed against CD2, CD16, CD19, CD36, CD56, CD66b, CD123, and glycophorin A on erythrocytes. One vial processes up to 50 mL of whole blood per separation, with isolation achievable in roughly 25 minutes at room temperature. Recovered cells remain surface-marker negative and are immediately compatible with downstream flow cytometry, PCR-based gene expression assays, mixed lymphocyte reactions, and in vitro culture expansion protocols.

STEMCELL Technologies manufactures this research-use-only single-use kit; the reagent is refrigerated and remains stable through the printed expiration date on the vial. Investigators commonly pair the reagent with Lymphoprep density medium and SepMate tubes to streamline the workflow. Typical applications include antigen-specific T cell response studies, viral immunity investigations, vaccine research pipelines, and preclinical adoptive immunotherapy development. The antibody cocktail is engineered to be human-specific, showing no significant cross-reactivity with rodent or non-human primate samples.