RosetteSep Human CD8 Depletion, 10mL

Deplete CD8+ cells directly from human whole blood with this antibody cocktail from STEMCELL Technologies. The reagent combines monoclonal antibodies in PBS that crosslink unwanted CD8+ cells to multiple red blood cells (RBCs), forming immunorosettes. During centrifugation over a density gradient medium, the rosetted cells pellet along with free RBCs, while unlabeled desired cells are easily collected as a highly enriched population at the plasma–density gradient interface. The workflow is fast and easy-to-use, requires no special equipment or training, and yields untouched, viable cells ready for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

The format can be combined with SepMate™ tubes for consistent, high-throughput sample processing, and the manufacturer's specifications indicate this format is intended for processing up to 200 mL of whole blood. For best results, peripheral blood should be used within 24 hours of collection and kept at room temperature (15–25°C); the nucleated cell concentration should not exceed 5 x 10^7 cells/mL, and the cocktail is added at 50 µL/mL of sample. After density gradient centrifugation at 1200 x g for 20 minutes with a standard tube or 10 minutes with SepMate, enriched cells are washed at 300 x g for 10 minutes. The cocktail is stored at 2–8°C and should not be frozen; it is stable until the expiration date on the label, and components may be shipped at room temperature (15–25°C). In the manufacturer's example, depletion achieved a 2.0 Log reduction, with residual CD8+ cells falling from 13% in the starting fraction to 0.4% in the depleted fraction as measured by flow cytometry using a sheep anti-mouse FITC secondary reagent. The product is for research use only.