RNeasy Midi Kit (50)

From QIAGEN, this kit provides fast purification of high-quality total RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns, and is regarded as a gold standard for total RNA isolation. It includes RNeasy Midi Spin Columns, Collection Tubes (15 ml), and RNase-free reagents and buffers, supporting purification from animal or human cells, animal or human tissues, and yeast.

The procedure starts with lysis and homogenization of the sample, followed by addition of ethanol to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane, where RNA binds and contaminants are efficiently washed away. For applications sensitive to very small amounts of DNA, residual DNA can be removed using a convenient on-column DNase treatment, and pure, concentrated RNA is eluted in water. RNA purified with this technology has A260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5), making it suitable for downstream applications including RNA-seq, quantitative real-time RT-PCR, real-time RT-PCR starting with as little as one cell, end-point RT-PCR, northern, dot, and slot blotting, array analysis, and Poly A+ RNA selection.