RNase A Solution (650 µl)

An endonuclease-free, quality-controlled reagent from QIAGEN, RNase A Solution is intended for the digestion of RNA during plasmid purification procedures. It is supplied at 4 mg/mL and is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA.

The enzyme is highly active across a wide range of reaction conditions and is difficult to inactivate. Unlike some preparations, this RNase A does not need to be boiled before use. Before use in plasmid and DNA purification, the solution should be centrifuged briefly and then added to Buffer P1 to reach a final concentration of 100 μg/mL, after which Buffer P1 should be stored at 2–8°C.

The reagent can also be applied to remove contaminating RNA from DNA samples after extraction or PCR, particularly when downstream applications such as cloning or sequencing require RNA-free DNA.