RNA Isolation Services - RNA Isolation Bacteria

Bacterial RNA extraction requires specialized lysis protocols to efficiently break rigid cell walls while preserving transcript integrity. This QIAGEN service delivers total RNA purified from submitted bacterial cultures in stabilized formats, supporting downstream applications such as RNA-Seq, transcriptomics, qRT-PCR, and microarray-based gene expression profiling. Samples are processed through enzymatic lysis using lysozyme combined with Proteinase K and mechanical disruption, followed by silica-membrane-based purification that removes genomic DNA contamination and protein residues. Quality control includes assessment of RNA Integrity Number (RIN), concentration measurement by spectrophotometry, and verification of A260/A280 absorbance ratios. Input ranges typically cover Gram-positive and Gram-negative species, with options for low-input preparations and custom scale-up to accommodate larger cell pellets. Deliverables include eluted RNA in nuclease-free buffer, shipped on dry ice and stored at -80 °C, accompanied by a comprehensive QC report. This outsourced workflow eliminates the need for in-house bead-beater instrumentation or hazardous phenol-chloroform extractions, suitable for core facilities, individual research groups, and clinical microbiology laboratories that require high-quality prokaryotic RNA without investing in dedicated bench infrastructure.