REPLI-g Single Cell Kit (24)

From QIAGEN, this kit is designed for highly uniform whole genome amplification (WGA) from single cells or limited sample material, including single bacterial or tumor cells (1 to <1000 cells) or purified genomic DNA. The system uses Multiple Displacement Amplification (MDA) technology with an optimized form of Phi 29 polymerase, delivering up to 1000-fold higher fidelity compared to Taq DNA polymerase and avoiding the sequence bias, locus dropouts, and errors associated with PCR-based WGA methods.

The kit includes REPLI-g sc Polymerase (capable of generating long fragments up to 70 kb), REPLI-g sc Reaction Buffer optimized for unbiased amplification and representation of all loci, and Buffer DLB for cell lysis and denaturation. All components undergo a controlled UV decontamination procedure to eliminate detectable residual DNA contamination. Gentle alkaline incubation keeps DNA fragmentation low during sample preparation. Typical DNA yields reach 40 µg regardless of starting template quantity, and amplified DNA shows an average product length of over 10 kb with maximized genome coverage, including fragments up to 100 kb without sequence bias.

Reaction setup requires approximately 15 minutes of hands-on time, with a total reaction time of 8–16 hours. Amplified DNA is suitable for downstream applications including next-generation sequencing (NGS), array comparative genomic hybridization (aCGH), Pyrosequencing, and real-time PCR analysis, and can be stored long-term at –20 °C.