REPLI-g Midi Kit (25)

QIAGEN's whole genome amplification kit uses Multiple Displacement Amplification (MDA) technology for unbiased amplification from small or limited samples, including purified genomic DNA, blood, and cells. The system relies on Phi29 DNA polymerase, which carries 3'→5' exonuclease proofreading activity and, together with exonuclease-resistant primers, achieves high-fidelity amplification and high DNA yield.

The REPLI-g Midi Kit delivers a typical yield of approximately 40 µg per 50 µL reaction. Average product length is typically greater than 10 kb, with a range between 2 kb and 100 kb. The isothermal amplification proceeds at 30°C for at least 8 hours, and gentle alkaline denaturation preserves nucleic acid integrity during the workflow. The method provides highly uniform amplification across the entire genome with negligible sequence bias.

Amplified DNA is suitable for downstream applications including whole genome sequencing, exome sequencing, SNP genotyping, mutation detection, real-time PCR, Sanger sequencing, and Pyrosequencing. Provided protocols cover amplification from purified genomic DNA and from blood or cells, with supplementary protocols available for dried blood cards, buccal cells, tissue, serum, plasma, and laser-microdissected cells. The kit is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of disease.